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custom-built inverted multiphoton microscope bruker fluorescence microscopy  (Bruker Corporation)


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    Bruker Corporation custom-built inverted multiphoton microscope bruker fluorescence microscopy
    Custom Built Inverted Multiphoton Microscope Bruker Fluorescence Microscopy, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+built+multiphoton+microscope/custom+built+inverted+multiphoton+microscope/pm40596459-579-13-15
    Average 90 stars, based on 1 article reviews
    custom-built inverted multiphoton microscope bruker fluorescence microscopy - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Systems and methods for sorting T cells by activation state
    Article Snippet: .. Fluorescence intensity and lifetime images were acquired using a custom-built multiphoton fluorescence microscope (Bruker Fluorescence Microscopy, Middleton, WI) adapted for fluorescence lifetime imaging with time-correlated single photon counting electronics. ..

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    Article Title: Naive primary neutrophils play a dual role in the tumor microenvironment
    Article Snippet: Kymograph and migrating speed of the immune cells were determined by using the kymograph and manual tracking plugin respectively in ImageJ. .. A custom-built inverted Ultima Multiphoton Imaging System (Bruker Fluorescence Microscopy, Middleton, WI) was used to acquire fluorescence intensity and lifetime images. .. The equipment consists of an ultrafast laser (Insight DS+, Spectra Physics), an inverted microscope (Nikon, Eclipse Ti), and a 40× water immersion 1.15 NA objective (Plan Apo, Nikon).

    Article Title: Metabolic changes in Toxoplasma gondii -infected host cells measured by autofluorescence imaging
    Article Snippet: For OMI, HFF dishes in metabolic media and in triplicate were treated as follows: (a) uninfected; (b) infected with 2 × 10 6 ME49 T. gondii tachyzoites; (c) infected with 2 × 10 6 ME49 tachyzoites that had been preincubated with 1.5 μM cytochalasin D during 15 minutes at 37°C (Sigma-Aldrich); (d) incubated with 1.5 μM cytochalasin D (Sigma-Aldrich). .. Fluorescence lifetime images were taken on a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI, USA), as previously described ( ). .. Fluorescence lifetime images were taken on a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI, USA), as previously described ( ).

    Article Title: Engineering the bone metastatic prostate cancer niche through a microphysiological system to report patient-specific treatment response.
    Article Snippet: Adipocytes were labeled with LipidSpot (Biotium) following the manufacturer’s instructions. .. Collagen hydrogel structure was visualized by second harmonic (SHG) using a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI), as described previously88. .. Plates were imaged using a Nikon TI Eclipse inverted microscope (Melville, New York), and devices were imaged in a Leica SP8 3X STED Super-resolution microscope (Wetzlar, Germany) in the UW-Madison Optical Imaging Core.

    Microscopy:

    Article Title: Systems and methods for sorting T cells by activation state
    Article Snippet: .. Fluorescence intensity and lifetime images were acquired using a custom-built multiphoton fluorescence microscope (Bruker Fluorescence Microscopy, Middleton, WI) adapted for fluorescence lifetime imaging with time-correlated single photon counting electronics. ..

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    Article Title: Naive primary neutrophils play a dual role in the tumor microenvironment
    Article Snippet: Kymograph and migrating speed of the immune cells were determined by using the kymograph and manual tracking plugin respectively in ImageJ. .. A custom-built inverted Ultima Multiphoton Imaging System (Bruker Fluorescence Microscopy, Middleton, WI) was used to acquire fluorescence intensity and lifetime images. .. The equipment consists of an ultrafast laser (Insight DS+, Spectra Physics), an inverted microscope (Nikon, Eclipse Ti), and a 40× water immersion 1.15 NA objective (Plan Apo, Nikon).

    Article Title: Metabolic changes in Toxoplasma gondii -infected host cells measured by autofluorescence imaging
    Article Snippet: For OMI, HFF dishes in metabolic media and in triplicate were treated as follows: (a) uninfected; (b) infected with 2 × 10 6 ME49 T. gondii tachyzoites; (c) infected with 2 × 10 6 ME49 tachyzoites that had been preincubated with 1.5 μM cytochalasin D during 15 minutes at 37°C (Sigma-Aldrich); (d) incubated with 1.5 μM cytochalasin D (Sigma-Aldrich). .. Fluorescence lifetime images were taken on a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI, USA), as previously described ( ). .. Fluorescence lifetime images were taken on a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI, USA), as previously described ( ).

    Article Title: Engineering the bone metastatic prostate cancer niche through a microphysiological system to report patient-specific treatment response.
    Article Snippet: Adipocytes were labeled with LipidSpot (Biotium) following the manufacturer’s instructions. .. Collagen hydrogel structure was visualized by second harmonic (SHG) using a custom-built inverted multiphoton microscope (Bruker Fluorescence Microscopy, Middleton, WI), as described previously88. .. Plates were imaged using a Nikon TI Eclipse inverted microscope (Melville, New York), and devices were imaged in a Leica SP8 3X STED Super-resolution microscope (Wetzlar, Germany) in the UW-Madison Optical Imaging Core.

    Imaging:

    Article Title: Systems and methods for sorting T cells by activation state
    Article Snippet: .. Fluorescence intensity and lifetime images were acquired using a custom-built multiphoton fluorescence microscope (Bruker Fluorescence Microscopy, Middleton, WI) adapted for fluorescence lifetime imaging with time-correlated single photon counting electronics. ..

    Article Title: Micro blood analysis technology (μBAT): multiplexed analysis of neutrophil phenotype and function from microliter whole blood samples.
    Article Snippet: There is an ongoing need to do more with less and provide highly multiplexed analysis from limited sample volumes.. Improved “sample sparing” assays would have a broad impact across pediatric and other rare sample type studies in addition to enabling sequential sampling.. This capability would advance both clinical and basic research applications.

    Article Title: Naive primary neutrophils play a dual role in the tumor microenvironment
    Article Snippet: Kymograph and migrating speed of the immune cells were determined by using the kymograph and manual tracking plugin respectively in ImageJ. .. A custom-built inverted Ultima Multiphoton Imaging System (Bruker Fluorescence Microscopy, Middleton, WI) was used to acquire fluorescence intensity and lifetime images. .. The equipment consists of an ultrafast laser (Insight DS+, Spectra Physics), an inverted microscope (Nikon, Eclipse Ti), and a 40× water immersion 1.15 NA objective (Plan Apo, Nikon).

    Counting:

    Article Title: Systems and methods for sorting T cells by activation state
    Article Snippet: .. Fluorescence intensity and lifetime images were acquired using a custom-built multiphoton fluorescence microscope (Bruker Fluorescence Microscopy, Middleton, WI) adapted for fluorescence lifetime imaging with time-correlated single photon counting electronics. ..

    Article Title: Autofluorescence lifetime imaging classifies human B and NK cell activation state
    Article Snippet: For imaging, cells were plated 1 h before imaging on poly-D-lysine coated glass-bottomed dishes (MatTek) at a seeding density of 200,000 cells in 50 μL media. .. The cells were imaged with a custom-built multiphoton fluorescence microscope (Ultima, Bruker) using a 100x (NA = 1.45) oil immersion objective and time-correlated single photon counting electronics (SPC-150, Becker and Hickl GbH, Berlin, Germany). .. The femtosecond-pulsed laser (Insight DS+, Spectra-Physics Inc., Santa Clara, CA, United States) with 80 MHz pulse repetition rate was tuned to 750 nm for NAD(P)H excitation, 890 nm for FAD excitation, and 980 nm or 1,040 nm excitation for PerCP.

    other:

    Article Title: Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation
    Article Snippet: Flow cytometry was performed on an Attune NxT (ThermoFisher Scientific), and data were analyzed using FCS Express.

    Article Title: Engineering the bone metastatic prostate cancer niche through a microphysiological system to report patient-specific treatment response
    Article Snippet: Plates were imaged using a Nikon TI Eclipse inverted microscope (Melville, New York), and devices were imaged in a Leica SP8 3X STED Super-resolution microscope (Wetzlar, Germany) in the UW-Madison Optical Imaging Core.



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